MAGUS Lum 400L Fluorescence Microscope

The MAGUS Lum 400L Fluorescence Microscope can be used for two microscopy techniques: fluorescence in reflected light and brightfield in transmitted light. When equipped with additional accessories, you can use the microscope to conduct research in polarized light as well as use darkfield and phase contrast techniques. The microscope can be used for research and laboratory diagnostics in the fields of medicine, pharmacology, forensics, biotechnology, veterinary medicine, etc.

Optics

The trinocular head includes two eyepiece tubes for observations and a trinocular tube for mounting a digital camera (not included). For comfortable longer observations, the eyepiece tubes can be adjusted to match the height of the observer, as they rotate 360°. They are 30° inclined.

The 5 objectives revolving nosepiece is oriented toward the interior – the user can see the objective inserted into the optical path. It comes with four infinity plan achromatic objectives: three for brightfield and one for fluorescence technique (fluo). One slot is left free when all objectives are installed in the revolving nosepiece. It is used for adjusting the position of the reflected light illuminator. You can also install an optional objective to get extra magnification.

Illumination

The reflected light – four 5W LEDs. Each LED has in a specific waveband: blue (B), green (G), violet (V), and ultraviolet (UV). To choose the working LED, you have to rotate the ring. The advantage of LED illumination is that LEDs get bright quickly and turn off quickly, making it easy to switch from brightfield to fluorescence and back again. LEDs do not heat up, they do not need a heat sink, and they have a lifetime of 50,000 hours without replacement. LED-based fluorescence is an easy to maintain and safe microscopy technique and, therefore, it is recommended for use at schools and universities.

The transmitted light is produced by a 3W LED. It also has a lifespan of 50,000 hours. The illumination system also includes a condenser. The condenser has a slot for a darkfield slider or a phase contrast slider. A slider is an easy and quick way to change the microscopy technique without changing the condenser.

For both transmitted and reflected light, you can set up Köhler illumination. The object of study is in sharp focus, and the image artifacts are removed. There is no darkening at the edges and the resolution limit can be reached at each objective.

Stage and focusing mechanism

The microscope stage is equipped with a removable specimen holder, which can be removed for manual scanning. The table does not have a positioning rack. Therefore, it requires less space for work. A black stage plate can be installed on the stage to eliminate stray light from the transmitted light source when observing in reflected light.

Coaxial coarse and fine focusing knobs are located at the base of the microscope on both sides. Coarse focusing has a lock knob and tension adjustment.

Accessories

The MAGUS Lum 400L microscope has a wide range of compatible accessories that can be used to expand its capabilities. It includes eyepieces, objectives, darkfield, polarized light and phase contrast devices as well as digital cameras and calibration slides.

Key features:

• Study of samples in reflected light using the fluorescence microscopy technique and in transmitted light using the brightfield microscopy technique.
• Infinity plan achromatic objectives: three brightfield objectives, one fluo objective
• Trinocular head, 360° rotatable barrels; a trinocular tube for mounting a digital camera
• Four reflected light LEDs operating at different wavelengths: blue (B), green (G), violet (V) and ultraviolet (UV)
• Transmitted light 3W LED, Köhler transmitted and reflected light illumination
• Stage without a positioning rack, removable specimen holder
• Wide range of accessories, including darkfield, polarized light and phase contrast devices

The kit includes:

• Base with a power input, transmitted light source and condenser, focusing mechanism, stage, and revolving nosepiece
• Reflected light illuminator
• Lamphouse with LEDs
• Trinocular head
• Infinity plan achromatic objective: PL 4x/0.10 WD 19.8mm
• Infinity plan achromatic objective: PL 10x/0.25 WD 5.0mm
• Infinity plan achromatic objective, fluo: PL FL 40x/0.85 (spring-loaded) WD 0.42mm
• Infinity plan achromatic objective: PL 100x/1.25 (spring-loaded, oil) WD 0.36mm
• Eyepiece 10x/22mm with long eye relief (2 pcs.)
• UV shield
• C-mount adapter 1x
• Hex key wrench
• Power supply unit for fluorescent LEDs
• Power cord
• Reflected light illuminator power cord
• Dust cover
• User manual and warranty card

Specifications

Product ID 82905

Brand MAGUS

Warranty 5

EAN 5905555018171

Package size (LxWxH) 45.2x30.2x96

Shipping Weight 14.5

Type biological, light/optical

Head trinocular

Nozzle Gemel head (Siedentopf, 360° rotation)

Head inclination angle 30 °

Magnification, x 40–1000 basic (*optional: 40–1250/1500/2000/2500)

Eyepiece tube diameter, mm 30

Eyepieces 10х/22mm, eye relief: 10mm (*optional: 10x/22mm with scale, 12.5x/14; 15x/15; 20x/12; 25x/9)

Objectives infinity plan achromatic and fluo objectives: PL 4x/0.10, PL 10x/0.25, PL FL 40x/0.85, PL 100x/1.25 (oil); parfocal distance 45mm (*optional: PL FL 10x/0.35, PL 60x/0.80 ∞/0.17)

Revolving nosepiece for 5 objectives

Working distance, mm 19.8 (4x); 5.0 (10x); 0.42 (FL 40x); 0.36 (100x); 2.37 (FL 10х); 0.46 (60х)

Interpupillary distance, mm 48 — 75

Stage, mm 180x150

Stage moving range, mm 75/50

Stage features two-axis mechanical stage, without a positioning rack

Condenser Abbe condenser, N.A. 1.25, center-adjustable, height-adjustable, adjustable aperture diaphragm, a slot for a darkfield slider and phase contrast slider, dovetail mount

Diaphragm adjustable aperture diaphragm, adjustable iris field diaphragm

Focus coaxial, coarse focusing (21mm, 39.8mm/circle, with a lock knob and tension adjusting knob) and fine focusing (0.002mm)

Illumination LED, fluorescent

Brightness adjustment yes

Power supply AC network, 85–265V, 50/60Hz

Light source type reflected light: 4 LEDs of different wavelengths, 5W; transmitted light: 3W LED

Ability to connect additional equipment phase contrast device (condenser and objectives), darkfield condenser (dry or oil), polarization devices (polarizer and analyzer), darkfield slider

User level experienced users, professionals

Assembly and installation difficulty level complicated

Fluorescent module filters: ultraviolet (UV), violet (V), blue (B), green (G)

Fluorescence filter: filter type, excitation wavelength/dichroic mirror/emission wavelength ultraviolet (UV), 320–380nm/420nm/435nm; violet (V), 380–415 nm/460 nm/475nm; blue (B), 410–490nm/505nm/515nm; green (G), 475–550nm/580nm/595nm

Application laboratory/medical

Illumination location dual

Research method bright field, fluorescence

Pouch/case/bag in set dust cover