Nikon P2-EFL Epi-FL-Illuminator (65490)

The Nikon P2-EFL Epi-FL-Illuminator is an epi-fluorescence attachment designed for advanced stereo microscopes like the Nikon SMZ25 and SMZ18. This illuminator enables fluorescence imaging by directing excitation light through the objective lens, allowing users to observe fluorescent signals with high clarity and contrast. Its fly-eye lens system ensures bright, uniform illumination across the entire field of view, making it suitable for both routine and advanced research applications in cell biology, developmental biology, materials science, and related fields. The P2-EFL can accommodate multiple filter cubes for different fluorophores, providing flexibility for a range of fluorescence experiments.

Purpose and Features:

• Allows epi-fluorescence microscopy by introducing excitation light through the objectives for detection of fluorescent signals

• Compatible with Nikon SMZ25 and SMZ18 stereo microscopes

• Holds up to four filter cubes, including options for GFP-B, GFP-L, and RFP, to support various fluorophores

• Fly-eye lens design provides even, high-intensity illumination across the sample area

• Can be used with high-performance light sources like the HG Precentered Fiber Illuminator Intensilight (C-HGFIE HG/C-HGFI HG, 130W)

• Includes a light shading plate to minimize stray light and enhance image contrast

• Available in manual and motorized versions, with the motorized model supporting remote operation and integration with Nikon NIS-Elements imaging software for automated workflows

• Compatible with additional illumination accessories, such as LED ring lights, coaxial illuminators, and fiber optic units

Operation:

• Insert the desired filter cube for your fluorophore into the illuminator

• Select and switch between filter cubes using the control box or software

• Adjust light intensity and ND filters for optimal image quality

• Close the shutter or block the light when not in use to prevent photobleaching

Applications:
Fluorescence imaging of cells, tissues, embryos, and other biological samples
Visualization of fluorescently labeled proteins, nucleic acids, or molecules
Research in developmental biology, neuroscience, and materials science
Routine laboratory imaging and advanced research requiring sensitive fluorescence detection

The Nikon P2-EFL Epi-FL-Illuminator is an essential tool for researchers needing reliable, high-contrast fluorescence imaging on Nikon stereo microscope systems, supporting a wide range of scientific and industrial applications.