Magus Epi-FL04M LED Epi-Fluorescence Attachment, fluorescence filter cubes B/G/UV (85153)

The epi-fluorescence attachment is designed to enable fluorescence microscopy. This technique relies on the ability of certain substances to emit light when excited by illumination at a specific wavelength. Some specimens naturally exhibit autofluorescence, while others require treatment with fluorochromes to produce a fluorescent signal.

The attachment is installed in the optical path between the microscope head and the stand. Inside the unit is a four-position rotating turret that holds three broadband fluorescence filter sets for blue (B), green (G), and ultraviolet (UV) excitation. The desired filter is placed into the optical path by pressing a control button. One button rotates the turret clockwise, while the other rotates it counterclockwise. One turret position is reserved for standard brightfield observation.

Excitation light source

Excitation is provided by LED light sources. The LEDs reach the selected brightness instantly, generate minimal heat even during extended operation, and can be switched off immediately when required. This allows fast and convenient switching between brightfield and fluorescence observation. The LED lifetime is approximately 50,000 hours. For educational environments, LED-based fluorescence is recommended because it is safe, reliable, and requires minimal maintenance.

Objectives for fluorescence

To perform fluorescence microscopy, the MAGUS Epi-FL04M LED attachment is used in combination with the MAGUS PlanF S-Apo fluorescent objective set. Plan semi-apochromatic fluorescent objectives increase resolving power and significantly reduce chromatic and spherical aberrations. Compared with standard plan achromatic objectives, they provide brighter images with higher contrast and improved clarity, both through the eyepieces and when using a digital camera.

Fluorescent objectives typically have a high numerical aperture, which improves resolution but reduces depth of field. For transmitted-light techniques, it is therefore recommended to use the standard objectives supplied with the microscope, as they provide a greater depth of field.

Compatibility

This epi-fluorescence attachment is compatible with the MAGUS Bio 260T biological microscope and the MAGUS Bio DH260 biological digital microscope.

Package contents

The package includes the epi-fluorescence attachment and a set of fluorescence filter cubes for blue, green, and ultraviolet excitation.

Specifications

Product ID: 85153
Brand: MAGUS
Warranty: 2 years
EAN: 5905555026176
Excitation light source: LEDs
Fluorescence filters:
– Blue (B): excitation 460–495 nm, dichroic mirror 505 nm, emission 510 nm
– Green (G): excitation 510–550 nm, dichroic mirror 570 nm, emission 575 nm
– Ultraviolet (UV): excitation 330–385 nm, dichroic mirror 410 nm, emission 420 nm