Magus Lum V500L Fluorescence Inverted Microscope (82909)

The MAGUS Lum V500L is an inverted microscope designed for fluorescence microscopy in reflected light and for brightfield and phase-contrast microscopy in transmitted light. Fluorescence observation is performed using filter sets for the DAPI, FITC, and TRITC fluorochromes. The inverted design allows specimens to be examined directly in common laboratory vessels such as Petri dishes and flasks. The maximum vessel height is 55 mm in the standard position, or up to 165 mm when the stand is tilted. The optical system is optimized for lab ware with a bottom thickness of 1.2 mm. This microscope is suitable for research, routine laboratory work, and educational use.

Optics

The microscope offers two connection options for imaging and display. The trinocular tube is intended for mounting a monitor (not included), while a side port on the microscope body is used for mounting a digital camera (not included). Beam-splitting options include 100/0 or 0/100 on the body, and 100/0 or 50/50 on the trinocular head. The eyepiece tubes are inclined at 45 degrees for comfortable viewing, and they can be rotated by 180 degrees during assembly.

The kit includes six infinity-corrected plan achromatic objectives with long working distances. Three objectives are intended for brightfield and fluorescence observation, and three are designed for phase-contrast work. A six-position revolving nosepiece located beneath the stage allows all objectives to be mounted and selected quickly.

Illumination

Fluorescence excitation is provided by a 5 W LED light source with an operating life of approximately 50,000 hours and no need for replacement. Three excitation filter sets are included for DAPI, FITC, and TRITC. LED illumination is energy efficient, delivers stable color output, switches on and off instantly, and does not overheat during extended use, making it well suited for universities and training laboratories.

Transmitted-light illumination is also provided by a 5 W LED source. It delivers sufficient brightness for both brightfield and phase-contrast observation with all supported objectives.

Both reflected and transmitted illumination systems can be adjusted using Köhler illumination. Proper Köhler setup helps achieve maximum resolution for each objective and produces a clear, evenly illuminated image without artifacts or edge darkening.

The microscope is equipped with a phase-contrast condenser turret. Three positions are dedicated to phase objectives with magnifications of 10x, 20x, and 40x, while the fourth position is used for brightfield. Phase rings can be centered, and switching between techniques is performed by rotating the control dial.

Stage and focusing

The fixed stage includes a mechanical holder for moving laboratory vessels and three cup holders in different sizes. The vessel can be moved smoothly in two perpendicular horizontal directions. The focusing system includes both coarse and fine adjustments. The coarse focus control includes a locking knob and tension adjustment. Ergonomically shaped controls help the user maintain a comfortable, relaxed posture during work.

Accessories

The microscope can be expanded with additional MAGUS eyepieces, calibration slides, and compatible digital cameras.

Key features

• Inverted design for lab ware with a 1.2 mm bottom thickness and vessel heights up to 55 mm, or up to 165 mm with the stand tilted

• Supported techniques: fluorescence in reflected light, brightfield and phase contrast in transmitted light

• Köhler illumination for both illumination paths, using long-life 5 W LED light sources

• Three fluorescence excitation filters for DAPI, FITC, and TRITC fluorochromes

• Phase-contrast condenser for fast switching between observation modes

• Trinocular head with two mounting options for a camera or monitor and flexible beam-splitting choices

Package contents

• Base with power input, reflected-light illuminator, transmitted-light source, condenser, focusing system, stage, and six-position revolving nosepiece

• Trinocular head

• Infinity plan achromatic objectives:

  • PLL 10x/0.25 phase, working distance 4.3 mm

  • PLL 20x/0.40, working distance 8.0 mm

  • PLL 40x/0.60, working distance 3.5 mm

  • PLL 10x/0.25 PHP2, working distance 4.3 mm

  • PLL 20x/0.40 PHP2, working distance 8.0 mm

  • PLL 40x/0.60 PHP2, working distance 3.5 mm

• Eyepieces 10x/22 mm with long eye relief, two pieces

• UV protection shield

• 1x C-mount adapter

• Hex key wrench

• Power cord

• Dust cover

• User manual and warranty card

Available on request

• 10x/22 mm eyepiece with scale

• Eyepieces: 12.5x/14 mm, 15x/15 mm, 20x/12 mm, 25x/9 mm

• Digital camera

• Calibration slide

Specifications

Product ID: 82909
Brand: MAGUS
Warranty: 5 years
EAN: 5905555018232
Package dimensions (L × W × H): 38 × 61 × 82 cm
Shipping weight: 24 kg

Type: Biological, optical microscope
Microscope head type: Trinocular
Head design: Siedentopf, rotatable 180 degrees
Magnification range: 100x to 400x, optional 40x to 1000x with additional components
Eyepiece tube diameter: 30 mm
Eyepieces: 10x/22 mm, eye relief 10 mm

Objectives: Infinity plan achromatic and phase objectives, parfocal distance 45 mm
Revolving nosepiece: Six-position
Working distances: 4.3 mm (10x), 8.0 mm (20x), 3.5 mm (40x)
Interpupillary distance: 48 to 75 mm

Stage size: 227 × 208 mm
Stage travel range: 77 × 134.5 mm
Stage type: Fixed stage with glass plate and mechanical attachment, multiple dish holders

Condenser: NA 0.6, working distance 55 mm, phase-contrast turret with locking screws
Diaphragm: Aperture iris diaphragm
Focusing: Coaxial coarse and fine focus knobs on both sides

Illumination: Dual illumination (reflected and transmitted)
Brightness adjustment: Available
Power supply: 85–265 V, 50/60 Hz AC
Light sources: Reflected light 5 W LED, transmitted light 5 W LED
Light filters: Available

Operating temperature range: +5 to +35 °C
User level: Experienced users and professionals
Assembly difficulty: Complicated

Fluorescent module: DAPI, FITC, and TRITC fluorochromes
Filter specifications:

• DAPI: excitation 365/35 nm, dichroic mirror 400 nm, emission 450/65 nm

• FITC: excitation 475/40 nm, dichroic mirror 500 nm, emission 535/45 nm

• TRITC: excitation 525/45 nm, dichroic mirror 565 nm, emission 595/60 nm

Application: Laboratory and medical use
Illumination location: Dual
Research methods: Brightfield, fluorescence, phase contrast
Included accessory: Dust cover