MAGUS Lum 400 Fluorescence Microscope

Fluorescence microscope MAGUS Lum 400 is designed for diagnostics and research by methods of fluorescence (reflected light) and brightfield (transmitted light) microscopy. Darkfield, phase contrast, and polarization techniques can be used with optional equipment. Areas of application of fluorescence microscope: DNA analysis, study of pathogens, sanitary and epidemiological surveillance, forensic examination, etc.

Optics

This is a trinocular microscope: The binocular head is 30° inclined and the digital camera (not included) is installed in the trinocular tube. As the eyepiece tubes are 360° rotatable, it is easy to adjust them to fit the height of the user.

Five objectives can be installed in the revolving eyepiece. Four infinity plan achromatic objectives are included. One of them, a fluo objective with a 40x magnification, is designed for fluorescence observations. 4x, 10x, and 100x objectives are used for the brightfield microscopy technique. An additional objective can be mounted in the free slot of the revolving nosepiece to achieve extra magnification within the magnification range of the microscope.

The revolving nosepiece with objectives is oriented toward the interior – the user can see the objective inserted into the optical path.

Illumination

The reflected light source is a 100W mercury lamp. It is placed inside the lamphouse, which provides efficient heat dissipation: The lamp does not overheat even during long hours of operation. The lamp can be centered along three axes to provide the optimal illumination of the working area. If you need to replace it, it will be fairly easy to remove and install the bulb. The mercury lamp is suitable for fluorescent dyes because it has discrete peaks. The reflected light illuminator contains four excitation filters: ultraviolet (UV), violet (V), blue (B), and green (G).

Transmitted light source: 30W halogen bulb. It is bright enough to work with any objectives of any magnification. Thanks to the color temperature of the illumination, even long observations will not strain the eyes. The illumination system also includes a condenser. It has a slot for a slider (phase contrast or darkfield).

In transmitted and reflected light, you can set up the Köhler illumination method. When the illumination is set up in this way, there is no darkening at the edges, the sample is clearly visible, and the image artifacts are removed.

Stage and focusing mechanism

The microscope is equipped with an ergonomically designed stage without a positioning rack. When observing in reflected light, a black stage plate is placed on the stage to remove stray light. The specimen holder is removable and can be taken out, if necessary, by loosening two screws. The focusing knobs are coaxial and located on each side of the microscope. Coarse focusing has a lock knob and tension adjustment and fine focusing is used for focus adjustment.

Accessories

The MAGUS Lum 400 microscope can be equipped with additional accessories: digital camera, phase contrast device, polarization device, darkfield condenser, eyepieces and objectives, darkfield and phase contrast sliders. For measuring objects, the configuration can be supplemented with a calibration slide.

Key features:

• Microscopy techniques: fluorescence in reflected light, brightfield in transmitted light
• Other research methods with optional accessories: phase contrast, simple polarization, darkfield
• Trinocular head with a trinocular tube for mounting a digital camera; 360° rotatable eyepiece tubes
• Infinity plan achromatic objectives, including a fluo objective
• Reflected light source: 100W mercury lamp, transmitted light source: 30W halogen lamp
• Köhler illumination method in transmitted and reflected light
• Fluorescence filters: ultraviolet (UV), violet (V), blue (B), green (G)
• Ergonomic stage with a removable specimen holder and no positioning rack

The kit includes:

• Base with a power input, transmitted light source and condenser, focusing mechanism, stage, and revolving nosepiece
• Reflected light illuminator
• Mercury lamphouse
• Trinocular head
• Infinity plan achromatic objective: PL 4x/0.10 WD 19.8mm
• Infinity plan achromatic objective: PL 10x/0.25 WD 5.0mm
• Infinity plan achromatic objective, fluo: PL FL 40x/0.85 (spring-loaded) WD 0.42mm
• Infinity plan achromatic objective: PL 100x/1.25 (spring-loaded, oil) WD 0.36mm
• Eyepiece 10x/22mm with long eye relief (2 pcs.)
• UV shield
• C-mount adapter 1x
• Hex key wrench
• Mercury lamp power supply
• Power cord
• Reflected light illuminator power cord
• Dust cover
• User manual and warranty card

Specifications

Product ID 82904

Brand MAGUS

Warranty 5

EAN 5905555018140

Package size (LxWxH) 45.2x30.2x96

Shipping Weight 16.5

Type biological, light/optical

Head trinocular

Nozzle Gemel head (Siedentopf, 360° rotation)

Head inclination angle 30 °

Magnification, x 40–1000 basic (*optional: 40–1250/1500/2000/2500)

Eyepiece tube diameter, mm 30

Eyepieces 10х/22mm, eye relief: 10mm (*optional: 10x/22mm with scale, 12.5x/14; 15x/15; 20x/12; 25x/9)

Objectives infinity plan achromatic and fluo objectives: PL 4x/0.10, PL 10x/0.25, PL FL 40x/0.85, PL 100x/1.25 (oil); parfocal distance 45mm (*optional: PL FL 10x/0.35, PL 60x/0.80 ∞/0.17)

Revolving nosepiece for 5 objectives

Working distance, mm 19.8 (4x); 5.0 (10x); 0.42 (FL 40x); 0.36 (100x); 2.37 (FL 10х); 0.46 (60х)

Interpupillary distance, mm 48 — 75

Stage, mm 180x150

Stage moving range, mm 75/50

Stage features two-axis mechanical stage, without a positioning rack

Condenser Abbe condenser, N.A. 1.25, center-adjustable, height-adjustable, adjustable aperture diaphragm, a slot for a darkfield slider and phase contrast slider, dovetail mount

Diaphragm adjustable aperture diaphragm, adjustable iris field diaphragm

Focus coaxial, coarse focusing (21mm, 39.8mm/circle, with a lock knob and tension adjusting knob) and fine focusing (0.002mm)

Illumination fluorescent, halogen

Brightness adjustment yes

Power supply AC network, 85–265V, 50/60Hz

Light source type reflected light: 100W mercury lamp; transmitted light: 12V/30W halogen lamp

Operating temperature range,°C 5...+35

Ability to connect additional equipment phase contrast device (condenser and objectives), darkfield condenser (dry or oil), polarization devices (polarizer and analyzer), darkfield slider

User level experienced users, professionals

Assembly and installation difficulty level complicated

Fluorescent module filters: ultraviolet (UV), violet (V), blue (B), green (G)

Fluorescence filter: filter type, excitation wavelength/dichroic mirror/emission wavelength ultraviolet (UV), 320–380nm/425 nm/435 nm; violet (V), 380–415nm/455nm/475nm; blue (B), 450–490nm/505nm/515nm; green (G), 495–555nm/585nm/595nm

Application laboratory/medical

Illumination location dual

Research method bright field, fluorescence

Pouch/case/bag in set dust cover